Journal: Neuron
Article Title: A Selectivity Filter Gate Controls Voltage-Gated Calcium Channel Calcium-Dependent Inactivation
doi: 10.1016/j.neuron.2019.01.011
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and Virus Strains Dh5α competent E. coli MCLAB Cat#DA-196 Chemicals, Peptides, and Recombinant Proteins ethylene glycol-bis(β-aminoethyl ether)-N,N,N’,N’-tetraacetic acid (EGTA) Sigma-Aldrich Cat#E4378 1,2-Bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid tetrakis (BAPTA) Santa Cruz Biotechnology Cat#sc-202076 Dulbecco’s modified Eagle’s medium (DMEM) GIBCO Cat#11965-092 fetal bovine serum (FBS) GIBCO Cat#16140-071 L-glutamine GIBCO Cat#25030-081 penicillin/streptomycin UCSF Cell Culture Facility Cat#CCFGK004-153K01 Lipofectamine 2000 Invitrogen Cat#11668-019 Matrigel BD Biosciences Cat#354234 Critical Commercial Assays T7 mMessenger kit Thermo Fisher Scientific Cat# AM1344 QuikChange Site-Directed Mutagenesis Kit Agilent Cat#200515 Experimental Models: Cell Lines HEK293 ATCC Cat#CRL-1573 Xenopus oocytes This study N/A Experimental Models: Organisms/Strains Xenopus Laevis Nasco Cat# LM00531 Recombinant DNA human Ca V 1.2/pcDNA3.1 Findeisen and Minor, 2009 N/A human Ca V 1.2 D707A/pcDNA3.1 Shaya et al., 2014 N/A human Ca V 1.2 D707N/pcDNA3.1 Shaya et al., 2014 N/A human Ca V 1.2 D707G/pcDNA3.1 Shaya et al., 2014 N/A human Ca V 1.2 D707E/pcDNA3.1 This study N/A human Ca V 1.2 E1115A/pcDNA3.1 This study N/A human Ca V 1.2 D367A/pcDNA3.1 This study N/A human Ca V 1.2 E1119A/pcDNA3.1 This study N/A human Ca V 1.2 D1420A/pcDNA3.1 This study N/A human Ca V 1.2 G364D/D707G/pcDNA3.1 This study N/A human Ca V 1.2 D707G/G1116D/pcDNA3.1 This study N/A human Ca V 1.2 D707A/A1417D/pcDNA3.1 This study N/A rat Ca V 1.3 42a /pcDNA6/V5-His ABC Xu and Lipscombe, 2001 Addgene#26577 rat Ca V 1.3 D726A/pcDNA6/V5-His ABC This study N/A rat Ca V 1.3 E1121A/pcDNA6/V5-His ABC This study N/A human Ca V 2.1/pcDNA3.1 Kim et al., 2008 N/A human Ca V 2.1 D667A/pcDNA3.1 This study N/A human Ca V 2.1 E1461A/pcDNA3.1 This study N/A rat Ca V β 2a /pTracer-CMV2-GFP Findeisen and Minor, 2009 N/A rabbit Ca V β 3 /pSport This study N/A rabbit Ca V α 2 δ-1/pcDNA3.1 Findeisen and Minor, 2009 N/A sv40 T-antigen/Bluescribe addgene Cat#21826 Software and Algorithms pCLAMP 9 Molecular Devices N/A Clampfit 10 Molecular Devices N/A SigmaStat 3.1 Systat N/A Open in a separate window KEY RESOURCES TABLE Ca V selectivity filter forms the calcium-dependent inactivation (CDI) endpoint Conserved Ca V domain II selectivity filter (+1) aspartate plays an active role in CDI Ca V selectivity filter asymmetry is important for CDI Ca V s gating relies on an SF-based gating framework shared among the VGIC superfamily
Techniques: Recombinant, Modification, Cell Culture, Mutagenesis, Software
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